Monocyte Activation Test (MAT) Hands-on Laboratory Training Course

Monocyte Activation Test (MAT)

Bernried, Germany

Seminar Nr. 20738

This course is part of the GMP Certification Programme "ECA Certified Microbiological Laboratory Manager". Learn more.

Participants of the “Low Endotoxin Recovery Laboratory Training Course” on 07/08 March 2023 in Bernried get a € 200 Discount.


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Rückfragen unter:
Tel.: 06221 / 84 44 0 E-Mail:


Jacqueline Dünisch, Labor LS
Dr Anja Fritsch, Confarma
Maria Gajewi, Microcoat
Dr Andreas Karst, Haemochrom Diagnostica
Dr Koen Marijt, MAT Research
Katrin Pauls, Lonza
Stéphanie Richard, Sanofi Pasteur
Dr Ruth Röder, Microcoat
Shabnam Solati, CTL-MAT
Dr Ingo Spreitzer, Paul-Ehrlich-Institut
Dr Sandra Stoppelkamp, Universität Tübingen and South Westphalia University of Applied Sciences


This unique event is organised to gain practical experience in performing the Monocyte Activation Test. Further, it brings together industry and regulatory representatives in order to discuss the practical experiences in using MAT, the advantages, the pitfalls, the available systems as well as the regulatory experiences. The purpose is to provide the participants with guidance and support and hands on laboratory training for the implementation and use of the MAT.


During recent years manifold advances have taken place to replace both, Rabbit Pyrogen Test (RPT) and in some cases the Limulus Amebocyte Lysate Test (LAL).

Some of the reasons are:
  • Animal experiments have to be reduced. Especially with the current EU Regulations.
  • Reduction of variance by validatable in-vitro test Methods
  • Get an alternative Test, where the classic LAL shows some gaps, e.g. Contamination of biologicals by non-endotoxin pyrogens not detectable in LAL test is not unlikely.
True In-vitro Pyrogen Tests (IVPT) have been developed in several European countries (United Kingdom, The Netherlands, Switzerland, Germany) in national and international research projects. The tests imitate the central step of human fever reaction, i.e. the activation of human monocytes by endotoxin as well as non-endotoxin pyrogens. One result of all these activities is the Monocyte Activation Test (MAT).

Following, with continuous support by the German Pharmacopoeia, the EDQM MAT Expert group was re-established and finally the MAT Monograph 2.6.30. was implemented into the European Pharmacopoeia in 2010. Meanwhile, the EDQM performed a MAT-survey to improve the MAT monograph and the BET-Guideline 5.1.10. and the Rabbit Pyrogen Test (RPT) monograph, 2.6.8. have been revised to clarify the priorisation of the MAT compared to the RPT (especially in regards to the EU directive 2010/63). However, there are still open questions how MAT can be applied in a routine lab for release testing. The meeting will give guidance for proper use of MAT and strengths and weaknesses will be discussed.


This course is addressed to all persons of
  •  pharmaceutical manufacturers
  •  biopharmaceutical companies
  •  medical device manufacturers
  •  contract laboratories
  •  tissue establishments
  •  authorities
who are involved in Endotoxin and Pyrogen Testing in development, IPC or release.

Presentations / Certificate / Hygiene Measures

The presentations for this event will be available for you to download and print before and after the event. Please note that no printed materials will be handed out on site and that there will not be any opportunity to print the presentations on site. After the event, you will automatically receive your certificate of participation.

On site, we will implement the necessary and required hygiene measures in close co-operation with the hotel. If infection rates and/or travel restrictions generally do not permit an on-site event, will be postponed or offered live online. In this case, you will be informed in due time.


Pyrogens – Fever inducing agents and the principal of the Monocyte Activation Test
  • What are pyrogens
  • Diversity of Pyrogens
  • Activation of the human immune system through TLRs Stimulation
  • Detection of cytokines using the MAT
MAT a compendial test method
  • Explanation of European Pharmacopoeia 2.6.30
  • International status of MAT
Pyrogen Testing - Fever in an animal‘s Body
  • History of the Rabbit Pyrogen Test
  • Pharmacopoeial Requirements
  • Regulatory Requirements and Animal Welfare (EU-Directive 2010/63/EU)
  • Field of Application
  • Future of Pyrogene Detection
Hands-on Training in the Laboratory
  •  Experimental set-up and preparation of samples (e.g., stimulation of cells)
  •  Loading of cells on assay plate
Background to Available Monocyte Activation Tests - Part 1
  • MAT from MAT Research
  • HaemoMAT® 
Multiple Read-out of Cytokines
  •  Available multiple read-out platforms
  •  Case study: Comparison of platforms
Recap of Day 1
Hands-on Training in the Lab
 Measurement of Samples

How to Monitor Pyrogen in Vaccines According to the General Chapter 2.6.30
  • Case Study 1 : Characterization of the absence of NEP
  • Case Study 2 : Consistency of production in the case of product containing intrinsic pyrogen
Generic Method and Specific Product Validation of the Monocyte Activation Test
Background to Available MATs - Part 2
  • PyroMAT
  • MAT Lonza/Sanquin
Hands-on Laboratory Results
  • Presentation of the laboratory results
  • Discussion of the results


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